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Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
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Biologia
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JoVE Journal Biologia
Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

74,946 Views

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07:10 min

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October 08, 2013

DOI:

10.3791/50684-v

DOI:
10.3791/50684-v

•

07:10 min
October 08, 2013

•

37 Views
, , ,
1Department of Biological Sciences,University of Texas Brownsville, 2Department of Medical Microbiology and Immunology,University of Alberta, 3Department of Biomedical Sciences,University of Texas Brownsville

Summary

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Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.

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ElectroporationElectrotransformationElectrocompetentE. ColiVibrio CholeraeCompetent BacteriaGene Pulse ControllerCuvettesRapid ProtocolTransformation Method

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Cite this Article

Gonzales, M. F., Brooks, T., Pukatzki, S. U., Provenzano, D. Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae. J. Vis. Exp. (80), e50684, doi:10.3791/50684 (2013).

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