Journal
/
生物学
/
Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

74,946 Views

•

07:10 min

•

October 08, 2013

DOI:

10.3791/50684-v

DOI:
10.3791/50684-v

•

07:10 min
October 08, 2013

•

37 Views
, , ,
1Department of Biological Sciences,University of Texas Brownsville, 2Department of Medical Microbiology and Immunology,University of Alberta, 3Department of Biomedical Sciences,University of Texas Brownsville

Summary

Automatically generated

Electroporation is a commonly employed method for introducing DNA into bacteria in a process known as transformation. Traditional protocols for the preparation of electrocompetent cells are time consuming and labor intensive. This article describes an alternate, rapid, and efficient method for the preparation of electrocompetent cells presently employed by some laboratories.

Explore More Videos

ElectroporationElectrotransformationElectrocompetentE. ColiVibrio CholeraeCompetent BacteriaGene Pulse ControllerCuvettesRapid ProtocolTransformation Method

Read Article

Cite this Article

Gonzales, M. F., Brooks, T., Pukatzki, S. U., Provenzano, D. Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae. J. Vis. Exp. (80), e50684, doi:10.3791/50684 (2013).

Download .ris file

Copy

Share Video

.

Copy