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Mise en place d'un<em> In vitro</em> Système d'étudier le comportement intracellulaire de<em> Candida glabrata</em> En droits macrophages THP-1
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
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Mise en place d'un<em> In vitro</em> Système d'étudier le comportement intracellulaire de<em> Candida glabrata</em> En droits macrophages THP-1

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13:47 min

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December 10, 2013

DOI:

10.3791/50625-v

DOI:
10.3791/50625-v

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13:47 min
December 10, 2013

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1Laboratory of Fungal Pathogenesis,Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent,Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium

Summary

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Le présent article décrit un protocole pour établir un système de modèle de culture cellulaire in vitro pour étudier l'interaction d'un champignon pathogène intracellulaire facultatif humain Candida glabrata avec les macrophages humains qui sera un outil utile pour faire progresser notre connaissance des mécanismes de virulence des champignons.

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Rai, M. N., Borah, S., Bairwa, G., Balusu, S., Gorityala, N., Kaur, R. Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages. J. Vis. Exp. (82), e50625, doi:10.3791/50625 (2013).

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