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Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

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10:32 min

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March 23, 2014

DOI:

10.3791/50620-v

DOI:
10.3791/50620-v

•

10:32 min
March 23, 2014

•

6 Views
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1Department of Pathology,Instituto Lauro de Souza Lima (ILSL), 2Laboratory Animal House,Instituto Lauro de Souza Lima (ILSL), 3Department of Microbiology,Instituto Lauro de Souza Lima (ILSL), 4Department of Pharmacology,Instituto Lauro de Souza Lima (ILSL)

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Mycobacterium LepraeLeprosyAthymic Nude MiceBacilli PurificationTrypsinZiehl-Neelsen StainingBacillary CountingFrozen Stock PreservationStrain Maintenance

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Trombone, A. P. F., Pedrini, S. C. B., Diório, S. M., Belone, A. d. F. F., Fachin, L. R. V., do Nascimento, D. C., Rosa, P. S. Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice. J. Vis. Exp. (85), e50620, doi:10.3791/50620 (2014).

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