Journal
/
Immunologie et infection
/
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice
Journal JoVE
Immunologie et infection
Author Produced
This content is Free Access.
Journal JoVE Immunologie et infection
Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

14,519 Views

•

10:32 min

•

March 23, 2014

DOI:

10.3791/50620-v

DOI:
10.3791/50620-v

•

10:32 min
March 23, 2014

•

7 Views
, , , , , ,
1Department of Pathology,Instituto Lauro de Souza Lima (ILSL), 2Laboratory Animal House,Instituto Lauro de Souza Lima (ILSL), 3Department of Microbiology,Instituto Lauro de Souza Lima (ILSL), 4Department of Pharmacology,Instituto Lauro de Souza Lima (ILSL)

Summary

Automatically generated

Mycobacterium leprae, the causative agent of leprosy, does not grow in vitro. We describe an easy to follow protocol to prepare a bacillary suspension to ensure the maintenance of large quantities of M. leprae for a variety of applications. Protocols for propagation by mouse footpad inoculation, evaluation of viability, freezing and thawing bacillary stock are described in detail.

Explore More Videos

Mycobacterium LepraeLeprosyAthymic Nude MiceBacilli PurificationTrypsinZiehl-Neelsen StainingBacillary CountingFrozen Stock PreservationStrain Maintenance

Read Article

Cite this Article

Trombone, A. P. F., Pedrini, S. C. B., Diório, S. M., Belone, A. d. F. F., Fachin, L. R. V., do Nascimento, D. C., Rosa, P. S. Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice. J. Vis. Exp. (85), e50620, doi:10.3791/50620 (2014).

Download .ris file

Copy

Share Video

.

Copy