Rivista
/
Immunologia e infezioni
/
Adenoviral transduktion af Naive CD4 T-celler til at studere Treg Differentiering
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral transduktion af Naive CD4 T-celler til at studere Treg Differentiering

DOI:
10.3791/50455-v

•

15:33 min

•

August 13, 2013

•

,
1Institute for Molecular Immunology,Helmholtz Zentrum München

Capitoli

  • 00:05Titolo
  • 02:03Cloning of a Gene of Interest into an Entry Vector
  • 03:16Generation of the Primary Virus Lysate
  • 05:47Virus Amplification
  • 07:34T Cell Infection
  • 09:01T Cell Activation and Polarization
  • 10:01T Cell Fixation Staining for Flow Cytometry
  • 11:51Infection Does Not Interfere with T Cell Activation
  • 14:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

Adenoviral genoverføring i naive CD4 T-celler med transgen ekspression af Coxsackie adenovirus receptoren muliggør molekylær analyse af regulatoriske T-celle differentiering<em> In vitro</em>.

Tags

Adenoviral TransductionNaive CD4 T CellsRegulatory T CellsTreg DifferentiationEctopic Gene ExpressionCoxsackie Adenovirus ReceptorTCR ActivationTGF-betaIL-2

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Retroviral Transduction of T-cell Receptors in Mouse T-cells

Directed Differentiation of Induced Pluripotent Stem Cells towards T Lymphocytes

Accelerated Type 1 Diabetes Induktion i mus ved adoptiv overførsel af diabetogene CD4 + T-celler

Isolering og Th17 Differentiering af Naïve CD4 T-lymfocytter

Transplantation af Tail Hud til Study Allogen CD4 T-celle respons i mus

Induktion af murine tarmbetændelse ved adoptiv overførsel af effektor CD4<sup> +</sup> CD45RB<sup> Høj</sup> T celler i immunsvækkede mus

Mus Naïve CD4<sup> +</sup> T Cell Isolation og<em> In vitro</em> Differentiering til T-celledelmængder

Isolering og<em> Ex vivo</em> Kultur Vδ1<sup> +</sup> CD4<sup> +</sup> γδ T-celler, en Extrathymic αβT-celleprogenitor

Retroviral transduktion af knoglemarven til at generere T-celle receptor Retrogenic Mus

Fremstilling af CD4<sup> +</sup> T-celler til analyse af GD3 og GD2 gangliosidet Membrane Expression ved mikroskopi

Read Article