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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

DOI:
10.3791/50455-v

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15:33 min

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August 13, 2013

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,
1Institute for Molecular Immunology,Helmholtz Zentrum München

Capitoli

  • 00:05Titolo
  • 02:03Cloning of a Gene of Interest into an Entry Vector
  • 03:16Generation of the Primary Virus Lysate
  • 05:47Virus Amplification
  • 07:34T Cell Infection
  • 09:01T Cell Activation and Polarization
  • 10:01T Cell Fixation Staining for Flow Cytometry
  • 11:51Infection Does Not Interfere with T Cell Activation
  • 14:42Conclusion

Summary

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Adenoviral gene transfer into naive CD4 T cells with transgenic expression of the Coxsackie adenovirus receptor enables the molecular analysis of regulatory T cell differentiation in vitro.

Tags

Adenoviral TransductionNaive CD4 T CellsRegulatory T CellsTreg DifferentiationEctopic Gene ExpressionCoxsackie Adenovirus ReceptorTCR ActivationTGF-betaIL-2

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