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Visualización y Análisis de las moléculas de ARNm mediante fluorescencia<em> In Situ</em> La hibridación en<em> Saccharomyces cerevisiae</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualización y Análisis de las moléculas de ARNm mediante fluorescencia<em> In Situ</em> La hibridación en<em> Saccharomyces cerevisiae</em

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

•

07:00 min
June 14, 2013

•

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, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Summary

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Este protocolo describe un procedimiento experimental para la realización de fluorescencia<em> In situ</em> La hibridación (FISH) para el recuento de ARNm en células individuales con una resolución de una sola molécula.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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