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Visualizzazione e l'analisi di molecole di mRNA mediante fluorescenza<em> In Situ</em> Ibridazione in<em> Saccharomyces cerevisiae</em
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Visualizzazione e l'analisi di molecole di mRNA mediante fluorescenza<em> In Situ</em> Ibridazione in<em> Saccharomyces cerevisiae</em

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07:00 min

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June 14, 2013

DOI:

10.3791/50382-v

DOI:
10.3791/50382-v

•

07:00 min
June 14, 2013

•

3 Views
, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Summary

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Questo protocollo descrive una procedura sperimentale per eseguire fluorescenza<em> In situ</em> Ibridazione (FISH) per il conteggio mRNA in cellule singole a risoluzione di una singola molecola.

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Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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Cite this Article

McIsaac, R. S., Silverman, S. J., Parsons, L., Xu, P., Briehof, R., McClean, M. N., Botstein, D. Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae. J. Vis. Exp. (76), e50382, doi:10.3791/50382 (2013).

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