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Neuroscience

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JoVE Journal Neuroscience
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
 

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Article DOI: 10.3791/50313-v • 08:39 min • March 18th, 2013
March 18th, 2013 •
1, 2, 3, 2
1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology, Columbia University, 3Department of Anesthesiology, Niigata University Graduate School of Medical and Dental Sciences

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Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.

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Stereotaxic Injection Viral Vector Conditional Gene Manipulation Mouse Spinal Cord Intraparenchymal Injection Targeted Gene Expression Silencing Dorsal Horn Surgical Procedure Laminectomy Quick Recovery Unimpaired Motility Controlled Injection Vector Suspension Volume Low Speed Microsyringe Beveled Glass Cannula Tissue Lesion Local Immune Response Recombinant Adeno-associated Virus Reporter Gene Enhanced Green Fluorescent Protein Spatial Distribution
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