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Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
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Neuroscienze
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JoVE Journal Neuroscienze
Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

16,405 Views

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19:45 min

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October 06, 2012

DOI:

10.3791/4093-v

DOI:
10.3791/4093-v

•

19:45 min
October 06, 2012

•

8 Views
, ,
1DFG – Research Center and Cluster of Excellence for Regenerative Therapies Dresden, Germany

Summary

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Controlling the expansion of somatic stem cells is a major factor hampering their study and use in therapy. Here we describe a system to temporally control neural stem cells expansion during development and adulthood, which can be used to increase the number of neurons generated in the mouse brain.

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Embryonic Neural Stem CellsAdult Neural Stem CellsIn Utero ElectroporationViral Stereotaxic InjectionSomatic Stem CellsRegenerative MedicineStem Cell ExpansionStem Cell DifferentiationCdk4/cyclinD1 ComplexG1 Phase Of The Cell Cycle

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Cite this Article

Artegiani, B., Lange, C., Calegari, F. Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection. J. Vis. Exp. (68), e4093, doi:10.3791/4093 (2012).

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