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实验方法测试的影响,在怀孕期间对酒精诱导的细胞凋亡在C57BL / 6小鼠神经营养肽,ADNF-9
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice
DOI:

09:50 min

April 24, 2013

Capitoli

  • 00:05Titolo
  • 01:26Alcohol Dosing and Trophic Peptide Treatment
  • 02:42Dissection of Fetuses and Microdissection of Fetal Brains
  • 04:32Embedding Fetal Brains in Gelatin for Sectioning
  • 06:07TUNEL Reaction for Detection of Apoptosis
  • 08:16Results: Effects of the Neurotrophic Peptide, ADNF-9 Against Alcohol-induced Apoptosis
  • 09:27Conclusion

Summary

Traduzione automatica

这里重点提出的实验设计,研究酒精暴露在怀孕期间胎儿大脑细胞凋亡和应用营养神经肽的影响。从养殖到胎儿大脑的集合的详细描述。细胞凋亡的测定技术进行详细说明。

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