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Experimental Methods for Testing the Effects of Neurotrophic Peptide, ADNF-9, Against Alcohol-induced Apoptosis during Pregnancy in C57BL/6 Mice
Chapters
- 00:05Title
- 01:26Alcohol Dosing and Trophic Peptide Treatment
- 02:42Dissection of Fetuses and Microdissection of Fetal Brains
- 04:32Embedding Fetal Brains in Gelatin for Sectioning
- 06:07TUNEL Reaction for Detection of Apoptosis
- 08:16Results: Effects of the Neurotrophic Peptide, ADNF-9 Against Alcohol-induced Apoptosis
- 09:27Conclusion
The experimental designs proposed here focus on studying the effects of alcohol exposure in apoptosis and the application of neurotrophic peptide during pregnancy in fetal brain. A detailed description from the breeding to the collection of fetal brains is described. Techniques for determination of apoptosis are also described in detail.
