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制备肿瘤抗原负载成熟树突状细胞免疫治疗
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
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制备肿瘤抗原负载成熟树突状细胞免疫治疗

18,052 Views

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08:40 min

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August 01, 2013

DOI:

10.3791/50085-v

DOI:
10.3791/50085-v

•

08:40 min
August 01, 2013

•

6 Views
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1Cancer Institute,NYU Langone Medical Center, 2Infectious Diseases,NYU Langone Medical Center

Summary

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用于产生在肿瘤免疫治疗中使用的大量的自体树突状细胞（DC）的最常用的方法进行说明。该方法使用的IL-4和GM-CSF分化的单核细胞的树突状。未成熟DC刺激才成熟，然后装入抗原注射到病人。

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Cite this Article

Sabado, R. L., Miller, E., Spadaccia, M., Vengco, I., Hasan, F., Bhardwaj, N. Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy. J. Vis. Exp. (78), e50085, doi:10.3791/50085 (2013).

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