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In ovo Expression of MicroRNA in Ventral Chick Midbrain
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09:19 min
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September 16, 2013
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Summary
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Ectopic expression is one technique to elucidate the microRNAs role in brain development. However, targeting specific areas using in ovo electroporation is challenging. Here, we show an efficient way to selectively electroporate ventral and dorsal midbrain regions.
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Huber, C., Prem Anand, A. A., Mauz, M., Künstle, P., Hupp, W., Hirt, B., Wizenmann, A. In ovo Expression of MicroRNA in Ventral Chick Midbrain. J. Vis. Exp. (79), e50024, doi:10.3791/50024 (2013).
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