Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
In ovo Expression of MicroRNA in Ventral Chick Midbrain
10,244 Views
•
09:19 min
•
September 16, 2013
DOI:
Summary
Automatically generated
Ectopic expression is one technique to elucidate the microRNAs role in brain development. However, targeting specific areas using in ovo electroporation is challenging. Here, we show an efficient way to selectively electroporate ventral and dorsal midbrain regions.
Read Article
Cite this Article
Huber, C., Prem Anand, A. A., Mauz, M., Künstle, P., Hupp, W., Hirt, B., Wizenmann, A. In ovo Expression of MicroRNA in Ventral Chick Midbrain. J. Vis. Exp. (79), e50024, doi:10.3791/50024 (2013).
Copy