Here we describe a unique strategy for creating biocompatible, layered matrices with continuous interfaces between distinct layers for tissue engineering. Such a scaffold could provide an ideal customizable environment to modulate cell behavior by various biological, chemical or mechanical cues
Joshi-Barr, S., Karpiak, J. V., Ner, Y., Wen, J. H., Engler, A. J., Almutairi, A. Density Gradient Multilayered Polymerization (DGMP): A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering. J. Vis. Exp. (72), e50018, doi:10.3791/50018 (2013).