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肺转移瘤在小鼠体内的组合在单细胞分辨率的可视化改进<em>在原位</em灌注肺组织和X-gal染色<em> lacZ的</em>标记的肿瘤细胞
JoVE Journal
Medicina
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JoVE Journal Medicina
Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
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肺转移瘤在小鼠体内的组合在单细胞分辨率的可视化改进<em>在原位</em灌注肺组织和X-gal染色<em> lacZ的</em>标记的肿瘤细胞

DOI:
10.3791/4162-v

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08:47 min

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August 21, 2012

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, ,
1Laboratory for Orthopedic Research,Balgrist University Hospital, Zurich

Capitoli

  • 00:05Titolo
  • 01:12In-situ Lung Perfusion and Fixation
  • 03:30Visualizing lacZ-tagged Metastatic Tumor Cells in Entire Lung Lobes
  • 04:31Visualizing lacZ-tagged Metastatic Tumor Cells in Lung Cryosections
  • 05:48Metastases Analysis in Dunn/LM8 Osteosarcoma-bearing Mice
  • 08:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

在本研究中报道的新颖协议在小鼠经合并在单细胞分辨率允许选择性检测肺转移<em>在原位</em>肺灌注固定和X-Gal染色<em> lacZ的</em>标记的肿瘤细胞。

Tags

Improved VisualizationLung MetastasesSingle Cell ResolutionMiceIn-situ PerfusionX-Gal StainingLacZ-tagged Tumor CellsMicrometastasesRadiologic ImagingTherapeutic EradicationAnimal ModelsRicerca sul cancroMonitoring/visualizationPreclinical StudiesClinical TrialsDetection TechniquesMicro-computer Tomography (micro-CT)Positron Emission Tomography (PET)Bioluminescence ImagingFluorescence ImagingOptimization TechniquesPrimary Tumor GrowthOsteosarcoma ModelsEx Vivo AnalysisQPCRFACSHistological Staining

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