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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube
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JoVE Journal Neuroscienze
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

16,952 Views

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12:48 min

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June 02, 2012

DOI:

10.3791/4134-v

DOI:
10.3791/4134-v

•

12:48 min
June 02, 2012

•

7 Views
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1Department of Cell and Developmental Biology, Center for Stem Cell Biology,Vanderbilt University Medical Center, 2Department of Pharmacology, Center for Stem Cell Biology,Vanderbilt University Medical Center, 3Vanderbilt University Medical Center

Summary

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Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.

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Pfaltzgraff, E. R., Mundell, N. A., Labosky, P. A. Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube. J. Vis. Exp. (64), e4134, doi:10.3791/4134 (2012).

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