Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Elise R. Pfaltzgraff; Nathan A. Mundell; Patricia A. Labosky

doi:10.3791/4134

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Neurowissenschaften

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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

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JoVE Journal Neurowissenschaften

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

16,952 Views

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12:48 min

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June 02, 2012

DOI:

10.3791/4134-v

DOI:

10.3791/4134-v

•

12:48 min

June 02, 2012

•

7 Views

Elise R. Pfaltzgraff¹, Nathan A. Mundell^1,2, Patricia A. Labosky³

¹Department of Cell and Developmental Biology, Center for Stem Cell Biology,Vanderbilt University Medical Center, ²Department of Pharmacology, Center for Stem Cell Biology,Vanderbilt University Medical Center, ³Vanderbilt University Medical Center

Summary

Isolation of embryonic neural crest from the neural tube facilitates the use of in vitro methods for studying migration, self-renewal, and multipotency of neural crest.

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Neural Crest Cells Embryonic Murine Neural Tube Isolation Culture Multipotent Progenitor Population Epithelial To Mesenchymal Transition Migration Self-renewal Avian Neural Tube Rat Systems Explanted NC Cells Proliferation Transplantation Cellular Properties Neural Tube Explant Assay Mammalian NC Isolation Methods Post-migratory NC Progenitors

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Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

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