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囊性纤维化跨膜传导调节蛋白表达和纯化酿酒酵母

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

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囊性纤维化跨膜传导调节蛋白表达和纯化酿酒酵母

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14:56 min

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March 10, 2012

DOI:

DOI:

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14:56 min

March 10, 2012

•

16 Views

Liam O’Ryan¹, Tracy Rimington¹, Natasha Cant¹, Robert C. Ford¹

¹Faculty of Life Sciences,University of Manchester

Summary

企图表达的囊性纤维化跨膜电导调节器（CFTR）<em>酿酒酵母</em>，到现在为止，已产生的蛋白质含量相对较低。通过囊性纤维化基金会分发本协议及相关的试剂应允许毫克这个'困难'真核生物膜蛋白的准备。

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