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Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
Kapitel
- 00:05Titel
- 03:42Screening Transformants
- 07:35Large-scale Fermenter Culture
- 11:37Representative Yeast Transformation Results
- 13:27Conclusion
Attempts to express the cystic fibrosis transmembrane conductance regulator (CFTR) in Saccharomyces cerevisiae have, until now, yielded relatively low amounts of protein. This protocol and the associated reagents distributed via the Cystic Fibrosis Foundation should allow the preparation of milligram amounts of this 'difficult' eukaryotic membrane protein.
Tags
Cystic Fibrosis Transmembrane Conductance RegulatorCFTR ProteinExpressionPurificationSaccharomyces CerevisiaeChloride ChannelCystic FibrosisProtein StructureProtein ActivityRecombinant ProteinYeast S. CerevisiaeProteolysis ProblemsCell Growth ConditionsInduction ConditionsReagentsUS Cystic Fibrosis Foundation
