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Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
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Medicina
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JoVE Journal Medicina
Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

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13:09 min

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April 04, 2012

DOI:

10.3791/3680-v

DOI:
10.3791/3680-v

•

13:09 min
April 04, 2012

•

2 Views
, , , , , , , ,
1Carestream Molecular Imaging , 2Department of Chemistry and Biochemistry,University of Notre Dame , 3Freimann Life Science Center,University of Notre Dame , 4Research and Development,Oncovision, GEM-Imaging S.A.

Summary

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Fat content analysis is routinely conducted in studies utilizing murine obesity models. Emerging methods in small animal CT imaging and analysis are providing for longitudinal detail rich fat content analysis. Here we detail step by step procedures for performing small animal CT imaging, analysis, and visualization.

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SegmentationMeasurementFat VolumesMurine Obesity ModelsX-ray Computed TomographyEnvironmental FactorsGenetic FactorsDisease MechanismsSmall Animal Models Of ObesityFat Content DataInvasive MethodsNon-invasive MethodsDual Energy X-ray Absorptiometry (DEXA)Magnetic Resonance (MR)Organ-specific Fat DistributionRegion-specific Fat DistributionLongitudinal AssessmentsSmall Animal CT Systems

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Sasser, T. A., Chapman, S. E., Li, S., Hudson, C., Orton, S. P., Diener, J. M., Gammon, S. T., Correcher, C., Leevy, W. M. Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography. J. Vis. Exp. (62), e3680, doi:10.3791/3680 (2012).

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