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简单而强大在体内和在体外方法研究病毒大会
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
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简单而强大在体内和在体外方法研究病毒大会

DOI:
10.3791/3645-v

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09:47 min

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March 01, 2012

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, , , ,
1Department of Plant Pathology and Microbiology,University of California, Riverside , 2Department of Bioengineering,University of California, Riverside

Capitoli

  • 00:05Titolo
  • 03:16Delivery and Expression of Functional Viral Genome Components to Plant Cells by Agroinfiltration
  • 06:00Preparation of Capsid Protein Subunits for in vitro Assembly
  • 07:07In vitro Assembly of RNA-containing Virions
  • 08:48Representative Viral Assembly Results
  • 09:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

同步提供多个病毒成分对植物细胞通过一种简单，高效和可靠的方法<em>农杆菌</em>介导的瞬时表达描述。这种方法是适合就读复制，包壳其次<em>在体外</em>到非病毒部分基因组的重组耗尽适用于生物医学应用光学病毒鬼。

Tags

Virus AssemblyIn VivoIn VitroEncapsidationPositive-sense RNA GenomesInfectious VirionsMechanism Regulating Virus AssemblyCurbing Virus SpreadDisease ControlMacromolecular InteractionsSubcellular CompartmentalizationBiomedical ImagingTherapeutic ApplicationsNon-enveloped Plant VirusesBrome Mosaic Virus (BMV)Genome PackagingNicotiana Benthamiana PlantsAgroinfiltration

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