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Neuroscience

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JoVE Journal Neuroscience
Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
 

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis

Article DOI: 10.3791/3606-v • 08:49 min • February 22nd, 2012
February 22nd, 2012 •
1, 1
1Center for Neural Repair and Rehabilitation, Temple University

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Automatic Translation

In this report we describe a method to crush mouse sciatic nerve. This method uses readily available hemostatic forceps and easily and reproducibly produces complete sciatic nerve crush. In addition, we describe a method to prepare muscle whole mounts suitable for analysis of nerve regeneration after sciatic nerve crush.

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Reproducible Mouse Sciatic Nerve Crush Regeneration Whole Mount Muscle Analysis Peripheral Nervous System PNS Neurons CNS Regeneration Axonotmesis Peripheral Nerve Injury Schwann Cell Basal Laminae Growing Axon Schwann Cell Interaction Basal Laminae Rat Models Experimental Nerve Crush Mouse Models Transgenic Lines Nerve Regeneration Nerve Crush Methods
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