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Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
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Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
Kapitel
- 00:05Titel
- 01:11Surgical Preparation
- 02:26Reproducible Sciatic Nerve Crush
- 05:17Whole Mount Muscle Preparation
- 06:59Assessment of Sciatic Nerve Regeneration by Whole Mount Muscle Analysis
- 08:31Conclusion
In this report we describe a method to crush mouse sciatic nerve. This method uses readily available hemostatic forceps and easily and reproducibly produces complete sciatic nerve crush. In addition, we describe a method to prepare muscle whole mounts suitable for analysis of nerve regeneration after sciatic nerve crush.
Tags
ReproducibleMouse Sciatic Nerve CrushRegenerationWhole Mount Muscle AnalysisPeripheral Nervous SystemPNS NeuronsCNS RegenerationAxonotmesisPeripheral Nerve InjurySchwann Cell Basal LaminaeGrowing AxonSchwann Cell InteractionBasal LaminaeRat ModelsExperimental Nerve CrushMouse ModelsTransgenic LinesNerve RegenerationNerve Crush Methods
