Rivista
/
Immunologia e infezioni
/
Murine Superficial Lymph Node Surgery
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Murine Superficial Lymph Node Surgery

Murine Superficial Lymph Node Surgery

DOI:
10.3791/3444-v

•

04:36 min

•

May 21, 2012

•

,
1Maisonneuve-Rosemont Hospital Research Center, 2Department of Microbiology and Immunology,University of Montreal, 3Department of Medicine,University of Montreal

Capitoli

  • 00:05Titolo
  • 00:53Murine Superficial Lymph Node Surgery
  • 03:53Results: LN Surgery is a Viable Method for Harvesting Lymphocytes
  • 04:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

Tags

MurineSuperficial Lymph Node SurgeryImmunologyImmune ResponseVaccineInfectionTumorLymphocyte HomeostasisTime Course ExperimentsExperimental VariabilityBlood WithdrawalCirculating LymphocytesLymph NodesSurgeryImmune Cell ExpansionPhenotypic CharacterizationFunctional AssaysBrachial Lymph NodesInguinal Lymph NodesSmall Incision In The SkinAnesthetized MouseQuick RecoveryMinimal Pain

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Intravital Microscopy of the Inguinal Lymph Node

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

Intravital Imaging of the Mouse Popliteal Lymph Node

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Isolation of Murine Lymph Node Stromal Cells

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin

Murine Full-thickness Skin Transplantation

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Read Article