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自动疏水层析蛋白质纯化用柱选择
JoVE Journal
Biologia
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JoVE Journal Biologia
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
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自动疏水层析蛋白质纯化用柱选择

42,533 Views

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10:21 min

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September 21, 2011

DOI:

10.3791/3060-v

DOI:
10.3791/3060-v

•

10:21 min
September 21, 2011

•

61 Views
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1College of Nursing, Interdisciplinary Life Sciences Research Laboratory,Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory,Seattle University

Summary

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提出了一个自动化的方法，确定合适的疏水作用层析（HIC）在蛋白质纯化过程中使用的媒体。该方法利用一个中型高压液相色谱系统包括自动缓冲混合，动态样本循环注射，顺序列选择，多波长分析，并分割部分洗脱液收集。

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Cite this Article

Murphy, P. J. M., Stone, O. J., Anderson, M. E. Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification. J. Vis. Exp. (55), e3060, doi:10.3791/3060 (2011).

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