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Ex Vivo Infection of Live Tissue with Oncolytic Viruses
Capitoli
- 00:05Titolo
- 02:00Tissue Processing
- 03:00Assessment of Tissue Viability
- 04:48Visualization of GFP Transgene Expression by Fluorescence Microscopy
- 05:50Visualization of Luciferase Expression using an In Vivo Imaging System (IVIS)
- 06:33Assessing Viral Titers by Plaque Assay
- 09:52Viability and Infectability of Patient Tissue Samples
- 11:07Conclusion
Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
