Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
Diallo, J., Roy, D., Abdelbary, H., De Silva, N., Bell, J. C. Ex Vivo Infection of Live Tissue with Oncolytic Viruses. J. Vis. Exp. (52), e2854, doi:10.3791/2854 (2011).