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小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
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小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

DOI:
10.3791/2785-v

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07:34 min

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June 02, 2011

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, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Capitoli

  • 00:05Titolo
  • 01:30Dissection of Mouse Femurs and Tibiae and Culture of Bone Marrow Cells
  • 03:59Collection and Labeling of DCs
  • 05:10Results: Qdot Nanocrystal Staining of DCs
  • 07:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

树突状细胞摄取抗原和迁移对目前处理的T细胞抗原的免疫器官。 Qdot纳米晶体的标签提供了一个长期和稳定的的荧光信号。这使得跟踪树突状细​​胞的荧光显微镜的不同器官。

Tags

GenerationLabelingMurineBone Marrow-derived Dendritic CellsQdot NanocrystalsTracking StudiesAntigen Presenting CellsImmunological OrgansMajor Histocompatibility MoleculesMigratory CapabilitiesFluorescent DyesQdot Fluorescent NanocrystalsStable FluorescenceCell CultureDifferentiationIn Vitro IncubationFluorescent MicroscopyExperimental AnimalsMature Cells

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