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Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
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Immunologie et infection
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Journal JoVE Immunologie et infection
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

24,602 Views

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07:34 min

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June 02, 2011

DOI:

10.3791/2785-v

DOI:
10.3791/2785-v

•

07:34 min
June 02, 2011

•

7 Views
, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Summary

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Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.

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Muccioli, M., Pate, M., Omosebi, O., Benencia, F. Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies. J. Vis. Exp. (52), e2785, doi:10.3791/2785 (2011).

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