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Mouse Model of Middle Cerebral Artery Occlusion
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JoVE Journal Medicina
Mouse Model of Middle Cerebral Artery Occlusion

Mouse Model of Middle Cerebral Artery Occlusion

DOI:
10.3791/2761-v

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09:43 min

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February 13, 2011

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, ,
1Department of Neurology,Ernest Gallo Clinic and Research Center, University of California, San Francisco, 2Department of Biological Sciences,Kent State University

Capitoli

  • 00:01Titolo
  • 00:22Preparing suture
  • 03:47Suture preparation
  • 09:00TTC Staining

Summary

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We demonstrate in the video a method for producing a middle cerebral artery occlusion in adult mice using an intraluminal monofilament. We also show how to evaluate the extent of cerebral infarction by 2,3,5-triphenyltetrazolium chloride (TTC) staining.

Tags

Mouse ModelMiddle Cerebral Artery Occlusion StrokeIschemic StrokeAnimal Stroke ModelsIntraluminal Monofilament ModelMCAOBlood Flow CessationBrain InfarctionReperfusionPermanent MCAOTransient MCAOCraniectomyCerebral IschemiaBrain Slices Staining

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