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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

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05:26 min

April 17, 2011

DOI:

05:26 min
April 17, 2011

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Summary

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We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.

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