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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
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April 17, 2011
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We demonstrate an in vivo electroporation protocol for transfecting single or small clusters of retinal ganglion cells (RGCs) and other retinal cell types in postnatal mice over a wide range of ages. The ability to label and genetically manipulate postnatal RGCs in vivo is a powerful tool for developmental studies.
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TransfectionMouse Retinal Ganglion CellsIn Vivo ElectroporationRGC ProjectionsNeural ConnectivityRetinofugal ProjectionsLateral Geniculate NucleusSuperior ColliculusDevelopmental ChangesAxonal Arbor MorphologyRetinotopic Map FormationGenetic ManipulationElectroporation ProtocolsPostnatal MiceLabeling Efficiency
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Dhande, O. S., Crair, M. C. Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation. J. Vis. Exp. (50), e2678, doi:10.3791/2678 (2011).
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