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Les analyses concurrentielles Homing d'étude Gut tropisme migration des lymphocytes T
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Competitive Homing Assays to Study Gut-tropic T Cell Migration
DOI:

10:25 min

March 01, 2011

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Capitoli

  • 00:05Titolo
  • 01:13Introduction
  • 01:59Ex vivo Generation of Gut-Homing and Control T cells
  • 04:25Analysis of Gut-Homing Receptors on Activated T cells
  • 05:21T cell Labeling with CFSE and CMTMR Cell Trackers
  • 07:42Analysis of T cell Homing
  • 08:58RA-induced Expression of a4B7 and CCR9 Direct T Cell Migration to the Gut
  • 09:50Conclusion

Summary

Traduzione automatica

Concurrentiels expériences homing permettent d'évaluer directement les propriétés migratoires des deux populations cellulaires différentes dans une seule souris. Ici, nous illustrons cette procédure en comparant la migration des ex vivo généré gut-tropic versus non-intestinale des cellules T tropiques.

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