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Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

DOI:

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11:45 min

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February 10, 2011

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Lisa Grant*¹, Eunyoung Yi*¹, Juan D. Goutman, Elisabeth Glowatzki

¹Department of Otolaryngology-Head and Neck Surgery.,The Johns Hopkins School of Medicine, ²Instituto de Investigaciones en Ingeniería Genética y Biología Molecular,Consejo Nacional de Investigaciones Científicas y Técnicas

Capitoli

00:05Titolo
01:08Prepare Tissue Holders and Electrodes
01:57Dissecting the Cochlear Tissue Sample
05:03Placing Electrodes for Inner Hair Cells and Afferent Boutons
08:43Results: Recordings from Afferent Fibers and Inner Hair Cells
11:26Conclusion

Summary

Traduzione automatica

Whole-cell patch-clamp recordings from auditory nerve fiber dendrites at the inner hair cell ribbon synapse in the mammalian cochlea.

Tags

Postsynaptic Recordings Afferent Dendrites Cochlear Inner Hair Cells Multivesicular Release Ribbon Synapse Auditory Nerve Fiber IHC Membrane Potential Excitatory Postsynaptic Currents (EPSCs)Presynaptic Ribbon Auditory Nerve Fibers Unmyelinated Single Ending Afferent Boutons Temporal Resolution Pre- And Postsynaptic Cells Neurotransmission

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