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Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse
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Neurociencias
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JoVE Revista Neurociencias
Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse

18,136 Views

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11:45 min

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February 10, 2011

DOI:

10.3791/2442-v

DOI:
10.3791/2442-v

•

11:45 min
February 10, 2011

•

3 Views
, , ,
1Department of Otolaryngology-Head and Neck Surgery.,The Johns Hopkins School of Medicine, 2Instituto de Investigaciones en Ingeniería Genética y Biología Molecular,Consejo Nacional de Investigaciones Científicas y Técnicas

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Whole-cell patch-clamp recordings from auditory nerve fiber dendrites at the inner hair cell ribbon synapse in the mammalian cochlea.

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Postsynaptic RecordingsAfferent DendritesCochlear Inner Hair CellsMultivesicular ReleaseRibbon SynapseAuditory Nerve FiberIHC Membrane PotentialExcitatory Postsynaptic Currents (EPSCs)Presynaptic RibbonAuditory Nerve FibersUnmyelinated Single EndingAfferent BoutonsTemporal ResolutionPre- And Postsynaptic CellsNeurotransmission

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Grant, L., Yi, E., Goutman, J. D., Glowatzki, E. Postsynaptic Recordings at Afferent Dendrites Contacting Cochlear Inner Hair Cells: Monitoring Multivesicular Release at a Ribbon Synapse. J. Vis. Exp. (48), e2442, doi:10.3791/2442 (2011).

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