We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
Chitrakar, R., Melotto, M. Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging. J. Vis. Exp. (44), e2185, doi:10.3791/2185 (2010).