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Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging
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October 02, 2010
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We have developed a simple and reproducible protocol to access stomatal response to live bacteria. This method minimizes wounding and manipulation of the leaf as compared to the use of epidermal peels reported previously.
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Stomatal ResponseLive Bacterial CellsWhole Leaf ImagingGuard CellsStomatal PoreGas ExchangeLight IntensityCarbon Dioxide ConcentrationRelative HumidityPathogen EntryDisease DevelopmentArabidopsis PlantsPlant Innate ImmunityEpidermal PeelsEnvironmental ConditionsMES BufferElectrophysiological ExperimentsBacterial Suspension
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Chitrakar, R., Melotto, M. Assessing Stomatal Response to Live Bacterial Cells using Whole Leaf Imaging. J. Vis. Exp. (44), e2185, doi:10.3791/2185 (2010).
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