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Primer-Free Seleção Aptamer usando uma biblioteca de DNA Aleatório
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July 26, 2010
DOI:
1Department of Pathology, Hershey Medical Center,Pennsylvania State University, 2Department of Chemistry,Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center,Pennsylvania State University, 4Materials Research Institute,Pennsylvania State University
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Primer-Free Aptamer SelectionRandom DNA LibraryAptamersOligonucleotidesDNARNAAffinitiesAntibodiesIn Vitro Selection ProcessSystematic Evolution Of Ligands By EXponential Enrichment (SELEX)TargetsSmall MoleculesProteinsMacromoleculesIn Vivo Diagnostic ApplicationsTherapeutic ApplicationsSpecificitySynthesizedProcessing ConditionsImmunogenicitySizeNucleotides (nt)Nucleic Acid LibrariesRandomized RegionsPrimer SitesSelection Process ComplicationsBioinformatics Approaches
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Pan, W., Xin, P., Patrick, S., Dean, S., Keating, C., Clawson, G. Primer-Free Aptamer Selection Using A Random DNA Library. J. Vis. Exp. (41), e2039, doi:10.3791/2039 (2010).
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