È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:08Titolo
- 00:45Introduction
- 01:14Post-fixing and Sectioning of Brain
- 01:55Pre-Embedding Immunocytochemistry
- 03:45Processing for Electron Microscopy
- 07:36Representative Images
- 08:20Conclusion
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
Tags
Immunoelectron MicroscopyTransmission Electron MicroscopyTEMMicroglialOligodendrocyticAstrocyticNeuronal Subcellular CompartmentsDendriteDendritic SpineAxonAxon TerminalPerikaryonIntracellular OrganellesCytoskeletonPre-embedding ImmunocytochemistryAntibodyIba1 MarkerNeurotransmitter ContentsUltrastructural Localization Of ProteinsAcrolein FixativeTranscardiac PerfusionBrain SectioningImmunoperoxidase-diaminobenzidine StainingResin EmbeddingUltrathin Sectioning
