Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
36,448 Views
•
08:47 min
•
July 20, 2010
DOI:
Summary
Automatically generated
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
Explore More Videos
Immunoelectron MicroscopyTransmission Electron MicroscopyTEMMicroglialOligodendrocyticAstrocyticNeuronal Subcellular CompartmentsDendriteDendritic SpineAxonAxon TerminalPerikaryonIntracellular OrganellesCytoskeletonPre-embedding ImmunocytochemistryAntibodyIba1 MarkerNeurotransmitter ContentsUltrastructural Localization Of ProteinsAcrolein FixativeTranscardiac PerfusionBrain SectioningImmunoperoxidase-diaminobenzidine StainingResin EmbeddingUltrathin Sectioning
Read Article
Cite this Article
Tremblay, M., Riad, M., Majewska, A. Preparation of Mouse Brain Tissue for Immunoelectron Microscopy. J. Vis. Exp. (41), e2021, doi:10.3791/2021 (2010).
Copy