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Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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July 20, 2010
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We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
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Immunoelectron MicroscopyTransmission Electron MicroscopyTEMMicroglialOligodendrocyticAstrocyticNeuronal Subcellular CompartmentsDendriteDendritic SpineAxonAxon TerminalPerikaryonIntracellular OrganellesCytoskeletonPre-embedding ImmunocytochemistryAntibodyIba1 MarkerNeurotransmitter ContentsUltrastructural Localization Of ProteinsAcrolein FixativeTranscardiac PerfusionBrain SectioningImmunoperoxidase-diaminobenzidine StainingResin EmbeddingUltrathin Sectioning
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Tremblay, M., Riad, M., Majewska, A. Preparation of Mouse Brain Tissue for Immunoelectron Microscopy. J. Vis. Exp. (41), e2021, doi:10.3791/2021 (2010).
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