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制备小鼠脑组织中的免疫电镜
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Neuroscienze
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JoVE Journal Neuroscienze
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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制备小鼠脑组织中的免疫电镜

DOI:
10.3791/2021-v

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08:47 min

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July 20, 2010

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, ,
1Department of Neurobiology and Anatomy,University of Rochester, 2Douglas Mental Health University Institute

Capitoli

  • 00:08Titolo
  • 00:45Introduction
  • 01:14Post-fixing and Sectioning of Brain
  • 01:55Pre-Embedding Immunocytochemistry
  • 03:45Processing for Electron Microscopy
  • 07:36Representative Images
  • 08:20Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述transcardiac灌注小鼠，拆除和切片的大脑，以及免疫过氧化物酶染色，树脂包埋，和脑切片切片超薄协议。这些程序完成后，免疫染色材料透射电子显微镜检查的准备。

Tags

Immunoelectron MicroscopyTransmission Electron MicroscopyTEMMicroglialOligodendrocyticAstrocyticNeuronal Subcellular CompartmentsDendriteDendritic SpineAxonAxon TerminalPerikaryonIntracellular OrganellesCytoskeletonPre-embedding ImmunocytochemistryAntibodyIba1 MarkerNeurotransmitter ContentsUltrastructural Localization Of ProteinsAcrolein FixativeTranscardiac PerfusionBrain SectioningImmunoperoxidase-diaminobenzidine StainingResin EmbeddingUltrathin Sectioning

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