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Menschliche T-Lymphozyten-Isolierung, Kultur und Analyse der Migration In Vitro</em
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Capitoli
- 00:00Titolo
- 00:37Introduction
- 01:31Isolation of Human T Lymphocytes
- 02:57Culture of Human T Lymphocytes
- 05:04In Vitro Lymphocyte Migration Assay
- 06:15Capturing an Image Sequence Using NIS Elements Software
- 07:20Representative Results
- 08:12Conclusion
T-Lymphozyten-Migration tritt bei der Referenzfahrt zu lymphatischen Organen, Ausfahrt aus dem Gefäßsystem, und das Eintreten in den peripheren Geweben. Hier beschreiben wir ein Protokoll, das verwendet werden, um T-Lymphozyten-Migration zu analysieren lassen<em> In-vitro-</em>.
Tags
T Lymphocyte IsolationCultureMigration AnalysisIntegrinsLigandsExtracellular Matrix ProteinsCell Leading EdgeUropodLeukocyte RecruitmentAutoimmune DiseasesInflammatory DiseasesIn Vitro SystemTwo-dimensional SubstrateIntercellular Adhesion Molecule-1 (ICAM-1)Integrin LFA-1Stromal Cell-derived Factor-1 (SDF-1)Migratory VelocityIntegrin BlockadeActomyosin Machinery
