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激光捕获显微切割技术的果蝇外周神经元
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JoVE Journal Neuroscienze
Laser Capture Microdissection of Drosophila Peripheral Neurons
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激光捕获显微切割技术的果蝇外周神经元

DOI:
10.3791/2016-v

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12:02 min

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May 24, 2010

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1Department of Molecular and Microbiology,George Mason University, 2Krasnow Institute for Advanced Study,George Mason University

Capitoli

  • 00:00Titolo
  • 01:18Introduction
  • 01:53Preparing the Larvae
  • 03:29Dehydration and Cuticle Removal from Frozen Larval Sections
  • 05:13Laser Capture Microdissection
  • 08:11RNA Isolation from LCM Derived Cells
  • 09:29Representative Results
  • 11:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这个视频文章中，我们目前的方法隔离单个或多个<em>果蝇</em> DA神经元使用红外线（IR）的捕获激光捕获显微切割（LCM）的类三龄幼虫。从孤立的神经元获得的RNA可以很容易地用于下游应用，包括QRT - PCR或芯片分析。

Tags

Laser Capture MicrodissectionDrosophila Peripheral NeuronsDendritic ArborizationDa NeuronsMolecular MechanismsClass-specific Dendrite MorphogenesisRNA Isolation TechniquesMagnetic Bead-based Cell PurificationFluorescent Activated Cell Sorting (FACS)RNA Binding Protein-based StrategiesLaser Capture Microdissection (LCM)Spatial PrecisionQRT-PCRMicroarray Expression ProfilingThird Instar Larval Stage

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