JOVE JOURNAL

Genetica

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Belle M. Sharon¹, Neha V. Hulyalkar¹, Vivian H. Nguyen¹, Philippe E. Zimmern², Kelli L. Palmer¹, Nicole J. De Nisco¹

¹Department of Biological Sciences,University of Texas at Dallas, ²Department of Urology,University of Texas Southwestern Medical Center

Journal (Genetics)

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira

L. G. V. Fernandes^1,2, R. L. Hornsby¹, A.L.T.O. Nascimento*², J. E. Nally*¹

¹Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service,United States Department of Agriculture, ²Laboratório de Desenvolvimento de Vacinas,Instituto Butantan

Journal (Genetics)

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Meike Siebers*¹, Agatha Walla*^1,2, Thea Rütjes¹, Moritz-Fabian Müller⁴, Maria von Korff^1,2,3

¹Institute of Plant Genetics,Heinrich-Heine-University, ²Cluster of Excellence on Plant Sciences "SMART Plants for Tomorrow's Needs", ³Max Planck Institute for Plant Breeding Research, ⁴IBG-1: Biotechnology, Forschungszentrum Jülich GmbH

Journal (Genetics)

Microinjection Method for Anopheles gambiae Embryos

Rebeca Carballar-Lejarazú¹, Taylor Tushar¹, Thai Binh Pham², Anthony A. James^1,2

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine

Journal (Genetics)

Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations

Rebeca Carballar-Lejarazú¹, Thai Binh Pham², Adam Kelsey¹, Taylor Tushar¹, Anthony A. James^1,2

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine

Journal (Genetics)

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Zechi Wu*¹, Wanyu Sun*¹, Sida Zhou¹, Li Zhang¹, Xinyu Zhao¹, Yang Xu¹, Weixiang Wang¹

¹Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy,Beijing University of Agriculture

Journal (Genetics)

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

Amanda O. Shaver¹, Goncalo J. Gouveia², Pamela S. Kirby³, Erik C. Andersen⁴, Arthur S. Edison^1,2,3

¹Department of Genetics,University of Georgia, ²Department of Biochemistry and Molecular Biology,University of Georgia, ³Complex Carbohydrate Research Center,University of Georgia, ⁴Department of Molecular Biosciences,Northwestern University

Journal (Genetics)

Small-Cage Laboratory Trials of Genetically-Engineered Anopheline Mosquitoes

Rebeca Carballar-Lejarazú¹, Thai Binh Pham², Vanessa Bottino-Rojas¹, Adriana Adolfi^1,3, Anthony A. James^1,2

¹Department of Microbiology & Molecular Genetics,University of California, Irvine, ²Department of Molecular Biology & Biochemistry,University of California, Irvine, ³Vector Biology Department,Liverpool School of Tropical Medicine

Journal (Genetics)

Using the GAL4-UAS System for Functional Genetics in Anopheles gambiae

Beth Crawford Poulton¹, Fraser Colman¹, Amalia Anthousi^1,2,3, Linda Grigoraki¹, Adriana Adolfi¹, Amy Lynd¹, Gareth John Lycett¹

¹Department of Vector Biology,Liverpool School of Tropical Medicine, ²IMBB FORTH, ³Department of Biology,University of Crete

Journal (Genetics)

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Camila F. Almeida¹, Emma C. Frair¹, Nianyuan Huang¹, Reid Neinast², Kim L. McBride^2,3,4,6, Robert B. Weiss⁵, Kevin M. Flanigan^1,6, Nicolas Wein^1,6

¹Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, ²Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, ³The Heart Center,Nationwide Children’s Hospital, ⁴Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, ⁵Department of Human Genetics,The University of Utah School of Medicine, ⁶Department of Pediatrics,The Ohio State University

Journal (Genetics)

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Kayli Sieber¹, Maya Saar¹, Comzit Opachaloemphan², Matthew Gallitto³, Huan Yang², Hua Yan^1,4

¹Department of Biology,University of Florida, ²Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ³Department of Radiation Oncology,Columbia University Medical Center, ⁴Center for Smell and Taste,University of Florida

Journal (Genetics)

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Adriana Adolfi^1,2, Amy Lynd¹, Gareth J. Lycett¹, Anthony A. James^2,3

¹Vector Biology Department,Liverpool School of Tropical Medicine, ²Department of Microbiology & Molecular Genetics,University of California, ³Department of Molecular Biology & Biochemistry,University of California

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