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Bio-ingénierie

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Efficient Adeno-Associated Virus Vector Production Using a Cell Factory Platform

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Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production

Journal JoVE
Bio-ingénierie

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Journal JoVE Bio-ingénierie

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform

Author Spotlight: Advancing Gene Therapy Research with High-Titer Adeno-Associated Virus Vector Production

Efficient Adeno-Associated Virus Vector Production Using a Cell Factory Platform

DOI:

10.3791/66741-v

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01:52 min

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May 03, 2024

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Ting Zhang, Vinitha Dhamotharan, Yu Xiao², Sydne Ballengee³, Jill Pollon⁴, George K. Gittes

¹Division of Pediatric Surgery, Department of Surgery, Children’s Hospital of Pittsburgh,University of Pittsburgh School of Medicine, ²North Allegheny High School, ³Wright State University Boonshoft School of Medicine, ⁴College of Engineering,University of Michigan

Tags

Adeno-associated Virus AAV Vectors Cell Factory Platform Cell Stacks HEK293T Cells Purification Yield Purity Polyethylene Glycol Iodixanol Gradient SDS-PAGE Transmission Electron Microscopy Gene Therapy Research Preclinical Studies

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