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Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms
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Neurosciences
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Journal JoVE Neurosciences
Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

DOI:
10.3791/65042-v

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08:28 min

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March 03, 2023

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, , , ,
1Department of Biomedical Engineering,Worcester Polytechnic Institute, 2Graduate School of Biomedical Sciences,UMass Chan Medical School, 3Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapitres

  • 00:04Introduction
  • 00:48Microfluidic Device Preparation
  • 02:04Animal Loading
  • 03:02Automated Stimulation and Neuronal Recording
  • 04:15Data Analysis Using NeuroTracker
  • 06:08Data Exploration and Visualization
  • 06:47Results: Assessment of Temporal Inhibition, Disinhibition, and Multimodal Stimulation
  • 07:51Conclusion

Summary

Traduction automatique

Traduction automatique

We present a method for the flexible chemical and multimodal stimulation and recording of simultaneous neural activity from many Caenorhabditis elegans worms. This method uses microfluidics, open-source hardware and software, and supervised automated data analysis to enable the measurement of neuronal phenomena such as adaptation, temporal inhibition, and stimulus crosstalk.

Tags

Automated StimulationMultimodal StimulationSimultaneous Neuronal RecordingMultiple Small OrganismsNeural ResponsesBrain ActivityRepeatabilityNeuronal VariabilityAdaptationSensory IntegrationMicrofluidic DeviceWorm LoadingNeural Phenomena

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